Vial of Human MMP-7
Protein

Human MMP-7 - 100 µL

$98.00
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  • Cat.Number : AS-72007
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    In stock
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-7 (matrilysin, PUMP-1) is proposed as a potential anti-cancer drug target.

Recombinant human MMP-7 was expressed in E.coli. The molecular mass of zymogen is approximately 28-kDa on SDS-PAGE and the active form is 19-kDa. pro-MMP-7 can be fully activated by incubating with 1 mM APMA at 37°C for 1 hr. Its activity can be measured by FRET peptides. 5-10 ng of enzyme is sufficient for FRET-based assay.

Specifications

Chemistry
UniProt number
  • P09237
Molecular Mass/ Weight
  • 19 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 10 µg/ml
Storage & stability
Form
  • In solution
Storage Buffer
  • 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM CaCl2, 1 mg/mL BSA.
Storage Conditions
  • Store at -80 ºC. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of protease hydrolyzes 1 picomole of Mca-Pro-Leu-Gly-LeuDap(Dnp)-Ala-Arg-NH2 per minute at pH 7.5 at 25° C
Specificity
  • Human MMP-7
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • human
Codes
Code Nacres
  • NA.27

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Citations

A bioresponsive hydrogel tuned to chondrogenesis of human mesenchymal stem cells

FASEB J . 2011 Jan 31 ; 25(5) 1486 | DOI : 10.1096/fj.10-165514

  • C. Bahney
  • et al