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PCR & qPCR
DNA Polymerases
DNA Polymerases
All reagent are in separated vials to allow you to tailor reaction mix to your needs.
A kit contains 3 tubes:
- Taq ® polymerase
- MgCl2
- 10X Buffer
RT-PCR
Reverse transcriptase can be done in a One-Step or a Two-Step approach
One-Step approach
- RT and PCR in a same tube
- Less contamination risks
- Less manipulation
Two-Step approach
- RT and PCR in separate tubes
- Ideal for rare sample or multiple targets
- Core kit including dNTPs
- Primers