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High performance probes
Access™ Dyes & Quenchers
With our Access™ Dyes and Quenchers, empower your assays with a class of proprietary molecules or others dyes & quenchers offering unmatched detection performance and IP-friendly terms and conditions.
A complete portfolio
covering all main qPCR Channels
Access Dyes and Quenchers include FAM, Yakima Yellow®, ROX, HiLyte Fluor™, EDQ, QXL® and others on request.
They span the full visible spectrum, from blue to far-red-shifted wavelengths.
Channels | Dye and Quencher pairs | Dye Emission λmax (nm) |
Quencher Abs λmax (nm) |
Order |
---|---|---|---|---|
● Deep Blue | Epoch Blue / DDQ1 | 460 | 440 | Request Now |
ATTO 390 / DDQ1 | 476 | 440 | View more | |
ATTO 425 / DDQ1 | 485 | 440 | View more | |
ATTO 465 / DDQ1 | 509 | 440 | View more | |
● Green Yellow | FAM / EDQ | 520 | 522 | View more |
● Yellow Orange | YY / EDQ | 549 | 522 | View more |
AP5 / EDQ | 549 | 522 | Request Now | |
HEX / EDQ | 556 | 522 | View more | |
YY / QXL570 | 549 | 538-577 | View more | |
● Orange Red | ROX / Q630 (Elitech) | 602 | 630 | Request Now |
ROX / QXL®610 | 602 | 594-628 | View more | |
AP593 / Q630 (Elitech) | 613 | 630 | Request Now | |
AP593 / QXL®610 | 613 | 594-628 | Request Now | |
● Red | HL647 / QXL®670 | 675 | 668 | View more |
● Infrared | HL750 / IRQXL® | 778 | 772 | View more |
High Performance Probes
Comparative performance of some dye & quencher pairs in qPCR application: for each channel, identical probes (24 bp each) were synthesized and
labeled with selected pairs of dyes and quenchers. The recommended Access™ Probe Pair is highlighted in orange.
Experiments were performed under standard conditions using Takyon™ No Rox Probe Mastermix dTTP from Eurogentec.
FAM/
EDQ Pair
Pair 1
Dye/Quencher performance comparison for FAM/TAMRA, FAM/BHQ1® and FAM/EDQ pairs. The recommended pair is in orange.
Yakima Yellow/
QXL®570 Pair
Pair 2
Dye/Quencher performance comparison for YY/EDQ, YY/BHQ1® and YY/QXL®570 pairs. The recommended pair is in orange.
ROX/
QXL® 610 Pair
Pair 3
Dye/Quencher performance comparison for ROX/DDQ2, ROX/BHQ2® and ROX/QXL®610 pairs. The recommended pair is in orange.
HiLyte Fluor™647/
QXL®670 Pair
Pair 4
Dye/Quencher performance comparison for Cy®5/QXL®670 and HiLyte™ Fluor 647/QXL®670 pairs. The recommended pair is in orange.
HiLyte Fluor™750/
IR-QXL®
Access™ IR Pair
Dedicated for use in Infrared channel.
Absorption and Emission spectra respectively for the IR-QXL® quencher and the HiLyte™ Fluor750 dye.
TRADEMARKS
BHQ® is a registered trademark of Biosearch Technologies, Inc. Cy® is a registered trademark of GE Healthcare.
Eclipse Dark Quencher is a trademark of ElitechGroup, Inc. Hilyte™ is a trademark of Anaspec,Inc.
QXL® is a registered trademark of Anaspec,Inc AquaPhluor® is a trademark of ElitechGroup, Inc.
Yakima Yellow® is a registered trademark of Epoch Biosciences, Inc.